Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients

Andika Aliviameita, Meryn Linanda Agustin, Anggie Wedha Puspita, Miftahul Mushlih, Puspitasari Puspitasari, Yanik Purwanti, Arief Wisaksono

Abstract

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.

Keywords

Salmonella typhi; Inv A gene; Fim C gene; multiplex PCR.

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