Condition Optimization for The Analysis of Risperidone and 9-0H-Risperidone by High-Performance Liquid Chromatography


  • Luki Yogaswara Yusuf Padjadjaran University Jenderal Achmad Yani University
  • Aliya Nur Hasanah Padjadjaran University
  • Melisa Intan Barliana Padjadjaran University



Risperidone, 9-OH-risperidone, clozapine, High-Performance Liquid Chromatography


Risperidone (RIS) is one of the most widely used atypical antipsychotics for treating schizophrenia in hospitals. RIS is metabolized by the liver and produces the primary active metabolite  9-OH-Risperidone (9-OHR). In the process of RIS metabolism, it is suspected that there are gene polymorphisms that cause variations in patient responses. Analysis of RIS and 9-OHR levels in the patient's blood can help to explain the various responses. High-Performance Liquid Chromatography (HPLC) is the most popular method to analyze RIS and 9-OHR, but many deficiencies were found in the chromatograms in the previous study. This research aims to obtain optimal conditions of the analysis prior to method validation. Condition optimization by optimizing the wavelength, composition of the mobile phase, pH, flow rate, and particle size of the stationary phase. The results showed that the wavelength was 279 nm, the mobile phase was 0.05 M KH2PO4 pH 3.7: acetonitrile (94:6, v/v) plus 0.3% triethylamine, and the flow rate was 1.2 ml/min in the stationary phase (LiChroCART® RP 18; 250x4 mm; 10 µm) being the optimal condition. This method is suggested to continue method validation for analyzing RIS and 9-OHR in the serum or plasma.

Author Biographies

Luki Yogaswara Yusuf, Padjadjaran University Jenderal Achmad Yani University

Magister Pharmacy Candidate at Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran University, Bandung, Jawa BaratLecturer with Work Aggrement at Department of Pharmaceutical Chemistry Analysis, Faculty of Pharmacy, Jenderal Achmad Yani University, Cimahi, Jawa Barat

Aliya Nur Hasanah, Padjadjaran University

Associate Professor, Vice Dean of Learning, Students Affairs and Research Pharmaceutical Analysis and Medicinal Chemistry Department, Faculty of Pharmacy, Universitas Padjadjaran

Melisa Intan Barliana, Padjadjaran University

Associate Professor, Department of Biological Pharmacy, Head of Master Program, Faculty of Pharmacy, Universitas Padjadjaran


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How to Cite

Yusuf, L. Y., Hasanah, A. N., & Barliana, M. I. (2022). Condition Optimization for The Analysis of Risperidone and 9-0H-Risperidone by High-Performance Liquid Chromatography. Medical Laboratory Technology Journal, 8(1), 12–19.