Effectiveness of Rosmarinus officinalis and Centella asiatica Nanoemulsions Against Caspase 3 Gestational Diabetes Mellitus Expression
DOI:
https://doi.org/10.31964/mltj.v9i2.537Keywords:
Caspase 3, Centella asiatica, gestational diabetes mellitus, nanoemulsion, Rosmarinus officinalisAbstract
Gestational diabetes mellitus (GDM) is closely related to oxidative stress conditions in insulin resistance conditions that increase Reactive Oxygen Species (ROS) against the body's defense antioxidant mechanism. Chronic complications due to hyperglycemia in patients with GDM increase BAX / BCL2 levels which then activates the change of procaspase 3 to caspase 3, an activator of apoptosis. This study aims to determine the effect of the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions on caspase 3 expressions in zebrafish models of gestational diabetes mellitus, with a posttest-only controlled group design. The samples were divided into five groups, namely K- (EM), K+(EM+3% Glucose), P1, P2 and P3 (3% Glucose + Combination of Rosmarinus officinalis and Centella asiatica 2.5μg/ml, 5μg/ml and 10μg/ml), which will be tested for PEPCK and Caspase expression at the age of 3dpf using Real Time-PCR. The results of the correlation test of the group with caspase 3 obtained (p = 0.045) negative direction. These results show that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsion decreased Caspase 3 expression in zebrafish models of gestational diabetes mellitus. The combination of Rosmarinus officinalis and Centella asiatica nanoemulsion has the potential to reduce blood glucose levels and reduce the risk of apoptosis in gestational diabetes mellitus patients.References
Baloch, J., Sohail, M. F., Sarwar, H. S., Kiani, M. H., Khan, G. M., Jahan, S., Rafay, M., Chaudhry, M. T., Yasinzai, M., & Shahnaz, G. (2019). Self-nanoemulsifying drug delivery system (Snedds) for improved oral bioavailability of chlorpromazine: In vitro and in vivo evaluation. Medicina (Lithuania), 55(5), 1–13. https://doi.org/10.3390/medicina55050210
Bao, T. Q., Li, Y., Qu, C., Zheng, Z. G., Yang, H., & Li, P. (2020). Antidiabetic effects and mechanisms of rosemary (Rosmarinus officinalis L.) and its phenolic components. American Journal of Chinese Medicine, 48(6), 1353–1368. https://doi.org/10.1142/S0192415X20500664
Chen, G. Q., & Lu, Y. (2011). Effector caspases and leukemia. International Journal of Cell Biology, 2011. https://doi.org/10.1155/2011/738301
Choudhury, A. A., & Devi Rajeswari, V. (2021). Gestational diabetes mellitus - A metabolic and reproductive disorder. Biomedicine & Pharmacotherapy, 143, 112183. https://doi.org/10.1016/J.BIOPHA.2021.112183
Ding, S., Anton, N., Akram, S., Er-Rafik, M., Anton, H., Klymchenko, A., Yu, W., Vandamme, T. F., & Serra, C. A. (2017). A new method for the formulation of double nanoemulsions. Soft Matter, 13(8), 1660–1669. https://doi.org/10.1039/c6sm02603f
Khotimah, H., Alita., Safira, N.A.P., Aninditha,D., Weningtyas, A., Wahyu P.E, Kalsum U., Rahayu, M., Handayani, D., & Nandar S.K. (2021). Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo. GSC Biological and Pharmaceutical Sciences, 16(3), 024–033. https://doi.org/10.30574/gscbps.2021.16.3.0213
Jusril, N. A., Abu Bakar, S. I., Khalil, K. A., Md Saad, W. M., Wen, N. K., & Adenan, M. I. (2022). Development and Optimization of Nanoemulsion from Ethanolic Extract of Centella asiatica (NanoSECA) Using D-Optimal Mixture Design to Improve Blood-Brain Barrier Permeability. Evidence-based Complementary and Alternative Medicine, 2022. https://doi.org/10.1155/2022/3483511
Keshavarzi, F., & Golsheh, S. (2019). IRS1- rs10498210 G/A and CCR5-59029 A/G polymorphisms in patients with type 2 diabetes in Kurdistan. Molecular Genetics and Genomic Medicine, 7(5), 1–7. https://doi.org/10.1002/mgg3.631
Manocha, S., Dhiman, S., Grewal, A. S., & Guarve, K. (2022). Nanotechnology: An approach to overcome bioavailability challenges of nutraceuticals. Journal of Drug Delivery Science and Technology, 72, 103418. https://doi.org/10.1016/J.JDDST.2022.103418
Martin-Piñero, M. J., Ramirez, P., Muñoz, J., & Alfaro, M. C. (2019). Development of rosemary essential oil nanoemulsions using a wheat biomass-derived surfactant. Colloids and Surfaces B: Biointerfaces, 173, 486–492. https://doi.org/10.1016/j.colsurfb.2018.10.024
Masola, B., Oguntibeju, O. O., & Oyenihi, A. B. (2018). Centella asiatica ameliorates diabetes-induced stress in rat tissues via influences on antioxidants and inflammatory cytokines. Biomedicine and Pharmacotherapy, 101(October 2017), 447–457. https://doi.org/10.1016/j.biopha.2018.02.115
Maulida, U., Jofrishal, & Mauliza. (2019). Uji Aktivitas Antidiabetes Ekstrak Etanol Pada Tanaman Pegegan (Centella asiatica (L) Urban. KATALIS Jurnal Pendidikan Kimia dan Ilmu Kimia, 2(2), 1–8.
Nabila, A., Puspitasari, C. E., & Erwinayanti, G. A. . S. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Rosemary (Rosemarinus officinalis L.) dengan Metode DPPH dan FRAP serta Pengaplikasiannya sebagai Zat Aktif dalam Losion. Jurnal Sains dan Kesehatan, 3(1), 242–247.
Naseema, A., Kovooru, L., Behera, A. K., Kumar, K. P. P., & Srivastava, P. (2021). A critical review of synthesis procedures, applications and future potential of nanoemulsions. Advances in Colloid and Interface Science, 287, 102318. https://doi.org/10.1016/J.CIS.2020.102318
Newsholme, P., Cruzat, V. F., Keane, K. N., Carlessi, R., & De Bittencourt, P. I. H. (2016). Molecular mechanisms of ROS production and oxidative stress in diabetes. Biochemical Journal, 473(24), 4527–4550. https://doi.org/10.1042/BCJ20160503C
Nieto, G., Ros, G., & Castillo, J. (2018). Antioxidant and Antimicrobial Properties of Rosemary (Rosmarinus officinalis, L.): A Review. Medicines, 5(3), 98. https://doi.org/10.3390/medicines5030098
Perkeni, (Persatuan Endokrinologi Indonesia) (2021). Pedoman Diagnosis Dan Penatalksanaan Hiperglikemia Dalam Kehamilan 2021. PB. Perkeni.
Plows, J. F., Stanley, J. L., Baker, P. N., Reynolds, C. M., & Vickers, M. H. (2018). The pathophysiology of gestational diabetes mellitus. International Journal of Molecular Sciences, 19(11), 1–21. https://doi.org/10.3390/ijms19113342
Puji, A. (2022). Mengenal Glukosa : Manfaat dan Cara Tubuh Mengolahnya. Accessed 10-08-2023. https://hellosehat.com/nutrisi/fakta-gizi/manfaat-glukosa/.
Rasoulian, B., Hajializadeh, Z., Esmaeili-Mahani, S., Rashidipour, M., Fatemi, I., & Kaeidi, A. (2019). Neuroprotective and antinociceptive effects of rosemary (Rosmarinus officinalis L.) extract in rats with painful diabetic neuropathy. Journal of Physiological Sciences, 69(1), 57–64. https://doi.org/10.1007/s12576-018-0620-x
Rita, C. I. (2020). Pengaruh Kombinasi Transfersom Pegagan dan Nanoemulsi Rosemary Terhadap Ekspresi Transforming Growth Factor Beta dan Matrix Metalloproteinase-9 Pada Proses Penyembuhan Luka Insisi Tikus (Rattus norvegicus). Universitas Brawijaya.
Rosmiati, K., & Fernando, A. (2018). Uji efektivitas antidiabetes ekstrak etanol daun ungu (Graptophyllum pictum) terhadap mencit putih jantan (Mus musculus). Jurnal Sains dan Teknologi Laboratorium Medik, 2(1), 8–13. https://doi.org/10.52071/jstlm.v2i1.14
Rueangdetnarong, H., Sekararithi, R., Jaiwongkam, T., Kumfu, S., Chattipakorn, N., Tongsong, T., & Jatavan, P. (2018). Comparisons of the oxidative stress biomarkers levels in gestational diabetes mellitus (GDM) and non-GDM among Thai population: cohort study. Endocrine Connections, 7(5), 681–687. https://doi.org/10.1530/EC-18-0093
Sari, L. M. (2018). Apoptosis: Mekanisme Molekuler Kematian Sel. Cakradonya Dental Journal, 10(2), 65–70. https://doi.org/10.24815/cdj.v10i2.11701
Seth, A., Stemple, D. L., & Barroso, I. (2013). The emerging use of zebrafish to model metabolic disease. DMM Disease Models and Mechanisms, 6(5), 1080–1088. https://doi.org/10.1242/dmm.011346
Singh, A., Castillo, H. A., Brown, J., Kaslin, J., Dwyer, K. M., & Gibert, Y. (2019). High glucose levels affect retinal patterning during zebrafish embryogenesis. Scientific Reports, 9(1), 1–13. https://doi.org/10.1038/s41598-019-41009-3
Sun, J., Singh, P., Österlund, J., Orho-Melander, M., Melander, O., Engström, G., & Edsfeldt, A. (2021). Hyperglycaemia-associated Caspase-3 predicts diabetes and coronary artery disease events. Journal of Internal Medicine, 290(4), 855–865. https://doi.org/10.1111/joim.13327
Tu, Z., Moss-Pierce, T., Ford, P., & Jiang, T. A. (2013). Rosemary (Rosmarinus officinalis L.) extract regulates glucose and lipid metabolism by activating AMPK and PPAR pathways in HepG2 cells. Journal of Agricultural and Food Chemistry, 61(11), 2803–2810. https://doi.org/10.1021/jf400298c
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Sutini Lamadi, Fajar Dwi Prastiwi, Tri Yudani Mardining Raras, Husnul Khotimah, I Wayan Arsana Wiyasa
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Publishing your paper with Medical Laboratory Technology Journal (MLTJ) means that the author or authors retain the copyright in the paper. MLTJ granted an author(s) rights to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by MLTJ in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.
MLTJ journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially. MLTJ journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:
BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.SA: If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
