Impact of Soybean Phytoestrogen-Rich Extract on Markers of Inflammation Markers in 4-Vinyl Cyclohexane Diepoxide-Induced Menopause in Albino Rats

Authors

  • Edusola Juliana Olaniyan Department of Medical Laboratory Science, University of Benin, Benin-City, Edo State, Nigeria
  • Mathias Abiodun Emokpae Department of Medical Laboratory Science, University of Benin, Benin-City, Edo State, Nigeria
  • Fidelis Ohiremen Oyakhire Department of Medical Laboratory Science, College of Health Sciences, Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State, Nigeria.
  • Ahmed Liasu Adeagbo Department of Family Medicine, Federal Medical Centre, Owo, Ondo State, Nigeria.
  • Iria Kelly Esezobor Department of Physiology, College of Health Sciences, Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State, Nigeria.
  • Stephen Olawale Olaniyan Department of Community Health, Federal Medical Centre, Owo, Ondo State, Nigeria.

DOI:

https://doi.org/10.31964/mltj.v9i2.549

Keywords:

4-vinyl cyclohexane diepoxide, inflammation, menopause, phytoestrogen

Abstract

The vasomotor symptoms of menopause, including hot flashes, sweating, physical and psychological discomfort, and emotional changes, are accurate and experienced by many of the menopausal and postmenopausal female population. In addition, it causes osteoporosis and slowed metabolism, raising the chance of developing many different ailments. Given that hormone replacement therapy (HRT) has been linked to an increased cancer risk, this investigation was undertaken to identify viable alternatives. The study aimed to investigate the impact of Soybean phytoestrogen-rich extract on some markers of inflammation of 4-vinyl cyclohexene diepoxide-induced menopause in albino rats. Sixty-five (65) female albino Wistar rats were employed in the investigation. Each one was induced with 80mg/kg of 4-vinyl cyclohexene diepoxide before being treated with either normal estradiol therapy (14ug/kg) or varying concentrations of the soybean phytoestrogen-rich extract (200 mg/kg, 400 mg/kg, and 600 mg/kg). Inflammatory markers (C-reactive protein (CRP), interleukin-6 (IL-6), and tumour necrosis factor-alpha (TNF-α)) were measured by ELISA methods. Statistical software SPSS (IBM) version 23.0 was used to analyze the data. Compared to the positive control group, the soybean phytoestrogen-rich extract therapy group saw a dose-dependent reduction in CRP and IL-6 levels (p<0.05) but not in TNF-α (P>0.05). Data from this research demonstrate the anti-inflammatory effects of soybean phytoestrogen-rich extract therapy in menopause-induced female Wistar rats. Soybean phytoestrogen-rich extract therapy in a high dose appears to show no significant difference from hormone replacement therapy as an alternate estrogen source in managing inflammation as a chronic disease.

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Published

2023-12-23

How to Cite

Olaniyan, E. J., Emokpae, M. A., Oyakhire, F. O., Adeagbo, A. L., Esezobor, I. K. ., & Olaniyan, S. O. (2023). Impact of Soybean Phytoestrogen-Rich Extract on Markers of Inflammation Markers in 4-Vinyl Cyclohexane Diepoxide-Induced Menopause in Albino Rats. Medical Laboratory Technology Journal, 9(2). https://doi.org/10.31964/mltj.v9i2.549

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