Invivo Effect Analysis of Rhodomyrtus tomentosa Leaf Ethanol Extract Against Escherichia coli

Doris Noviani, Adisty Dwi Treasa, Ade Zakiya Tasman Munaf, Sri Winarsih, Dwi Yuni Nur Hidayati, Mukhamad Nooryanto, Sutrisno Sutrisno

Abstract

Puerperium infection contributed 11 percent of 48.17 percent of maternal mortality during the puerperium period; one of the causes of increased risk of puerperium infection was bacteria Gram-negative Escherichia coli. Puerperium infection occurred because bacteria enter through the vagina, wounds on the insertion of the placenta, and other parts spread to blood throughout the body and damage the spleen, liver, and uterus. This research aimed to determine the effect of Rhodomyrtus tomentosa leaves ethanol extract (Rtl-EE) as an antibacterial on the spleen, liver, and uterus of Escherichia coli -induced puerperal infection model mice. Mice in the postnatal treatment group were induced by Escherichia coli bacteria intravaginally and given different doses of ethanol extract of Rhodomyrtus tomentosa leaves. Examination of bacterial colonies growth used pour plate method with EMBA media. The research results showed that the administration of Rtl-EE decreased the growth of bacterial colonies respectively in the liver 1280, 924, 330 CFU/mL; the uterus 1806, 1180, 874 CFU/mL; and the spleen 712, 112, 774 CFU/mL. Colony growth started to decrease at dose 1 of Rtl-EE (100mg/kg BW) of mice model of Escherichia coli -induced puerperal infection. Rhodomyrtus tomentosa leaves contain active compounds - phenols, flavonoids, saponins, tannins, alkaloids, and triterpenoids as antibacterial. Rtl-EE had an antibacterial effect by reducing the number of bacteria on the spleen, liver, and uterus of Escherichia coli -induced puerperal infection model mice. Further research needs to conduct to examine the toxic dose of Rhodomyrtus tomentosa leaves against Escherichia coli bacteria.

Keywords

Rhodomyrtus tomentosa; puerperium infections; Escherichia coli

Full Text:

PDF

References

Boushra, M., & Rahman, O. (2020). Postpartum Infection. StatPearls Publishing.

Cunningham, F. G., Leveno, K. J., Bloom, S. L., Hauth, J., Rouse, D., & Spong, C. Y. (2010). Puerperal Infection. In Williams Obstetrics 23rd Edition. Mc Graw Hill Co.

Darsana, I. G. O., Besung, I. N. K., & Mahatmi, H. (2012). The Potential of Binahong Leaves (Anredera Cordifolia (Tenore) Steenis) in Inhibiting the Growth of Escherichia coli Bacteria, Indonesia Medicus Veterinus, 1(3).

Dwicahmi, P. (2015). Antibacterial activity test of 70% ethanol extract of karamunting (Rhodomyrtus tomentosa (Ait.) Hassk) leaves on the growth of Vibrio cholerae bacteria in vitro, Tanjungpura Pontianak University.

Harlia Erika Juniar, A. H. A. (2017). Cytotoxic and Antioxidant Activity of Karamunting Stem Extract (Rhodomyrtus Tomentosa (Aiton) Hassk), Journal of Equatorial Chemistry, 6(2), pp. 37–43.

Haruna, N. (2017). Mother's knowledge and attitude about prevention and control of puerperal infection in Bella Bori and Bella Punranga, Regency of Gowa, Experience Journal, 1(1), pp. 67–69.

Hilda and Berliana. (2015), Patterns of resistance of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa bacteria to various antibiotics in the Health Laboratory of East Kalimantan Province in 2013, Journal of Laboratory Technology,4(2).

Indriani, O., Fatiqin, A., & Oktarina, T. (2019). Effect of Extract and Fraction of Karamunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk.) on the Growth of Escherichia coli Bacteria, Jurnal Aisyiyah Medika, 4(3).

Ismandari, T., Kumalaningsih, S., Wijana, S., & Mustaniroh, S. A. ul. (2020). Optimization of Bioactive Compound Extraction from Rose Myrtle Fruit (Rhodomyrtus tomentosa, (W.Ait), Myrtaceae) as the Antioxidant Source, Scientific World Journal.

Limsuwan, S., Hesseling-Meinders, A., Voravuthikunchai, S. P., van Dijl, J. M., & Kayser, O. (2011). Potential antibiotic and anti-infective effects of rhodomyrtone from Rhodomyrtus tomentosa (Aiton) Hassk. on Streptococcus pyogenes as revealed by proteomics, Phytomedicine, 18(11), 934–940.

Marni. (2012). Midwifery Care in Childbirth. Yogyakarta: Student Library.

Megawati, E. P., Khotimah, S., & Bangsawan, P. I. (2016). Antifungal Activity Test of Karamunting leaves (Rhodomyrtus tomentosa) Ethanol Extract Against the Growth of Candida Albicans In Vitro, Students Journal PSPD FK Tanjungpura University.

Mochtar R, dan S. A. (2012). inopsis Obstetri: Obstetri Fisiologi Obstetri Patologi Edisi III. EGC.

Mordmuang, A., & Voravuthikunchai, S. P. (2015). Rhodomyrtus tomentosa (Aiton) Hassk. leaf extract: An alternative approach for the treatment of staphylococcal bovine mastitis, Research in Veterinary Science, 102, 242–246.

Pascual, L., Ruiz, F., Giordano, W., & Barberis, I. L. (2010). Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection, Journal of Medical Microbiology, 59(3), 360–364.

Sáez-López, E., Guiral, E., Fernández-Orth, D., Villanueva, S., Goncé, A., López, M., Teixidó, I., Pericot, A., Figueras, F., Palacio, M., Cobo, T., Bosch, J., & Soto, S. M. (2016). Vaginal versus Obstetric Infection Escherichia coli Isolates among Pregnant Women: Antimicrobial Resistance and Genetic Virulence Profile, PLoS One, 11(1).

Saising, J., Ongsakul, M., & Voravuthikunchai, S. P. (2011). Rhodomyrtus tomentosa (Aiton) Hassk. ethanol extract and rhodomyrtone: a potential strategy for the treatment of biofilm-forming staphylococci, Journal of Medical Microbiology, 60(Pt 12), 1793–1800.

Senthilmurugan G, Vasanthe B, Suresh K. (2013). Screening and antibacterial activity analysis of some important medicinal plants, Intl J Innov Appl Stud 2: 146-152.

Song, H., Hu, K., Du, X., Zhang, J., & Zhao, S. (2020). Risk factors, changes in serum inflammatory factors, and clinical prevention and control measures for puerperal infection, Journal of Clinical Laboratory Analysis, 34(3), 1–7.

Sukeri, S., Karem, A.A., Kamarudin, E. and Bahari, M., (2021). Antimicrobial Activity of Methanolic and Aqueous Extract of Rhodomyrtus tomentosa Leaves against Staphylococcus aureus and Escherichia coli, J Pure Appl Microbiol, 15(1), pp186-19.

Wardhani, R. K., Sumarno, & Endharti, A. T. (2017). Effect of probiotic lactobacillus reuteri on the percentage of regulatory cells and T helper cells 22 in the spleen of puerperal mice induced by Staphylococcus aureus bacteria, Journal of Issues in Midwifery, 1(3), pp. 18–29.

Refbacks

  • There are currently no refbacks.