Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility

Authors

  • Silvani Permatasari Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia
  • Nida Halisa Bachelor degree of Medical, Faculty of Medicine, Universitas Palangka Raya, Indonesia
  • Agnes Frethernety Department of Pharmacotherapy, Faculty of Medicine, University of Palangka Raya, Indonesia

DOI:

https://doi.org/10.31964/mltj.v9i2.563

Keywords:

Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb), spermatozoa, motility

Abstract

Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.

References

Assidi, M. (2022). Infertility in Men: Advances towards a Comprehensive and Integrative Strategy for Precision Theranostics. Cells, 11(10), 1711. https://doi.org/10.3390/cells11101711

Boitrelle, F., Shah, R., Saleh, R., Henkel, R., Kandil, H., Chung, E., Vogiatzi, P., Zini, A., Arafa, M., & Agarwal, A. (2021). The Sixth Edition of the WHO Manual for Human Semen Analysis: A Critical Review and SWOT Analysis. Life, 11(12), 1368. https://doi.org/10.3390/life11121368

Charit, A. B., & Sirait, B. I. (2020). Jumlah Sperma Motil Yang Memberikan Keberhasilan Tertinggi Pada Inseminasi Intra Uterin di Morula IVF Jakarta Periode Juni-Oktober 2018. Jurnal Ilmiah WIDYA Kesehatan Dan Lingkungan, 1(3), 221–228.

Cimini, C., Ramal-Sanchez, M., Taraschi, A., Della Pelle, F., Scroccarello, A., Belda-Perez, R., Valbonetti, L., Lanuti, P., Marchisio, M., D’Atri, M., Ortolani, C., Papa, S., Capacchietti, G., Bernabò, N., Compagnone, D., & Barboni, B. (2023). Catechin versus MoS2 Nanoflakes Functionalized with Catechin: Improving the Sperm Fertilizing Ability—An In Vitro Study in a Swine Model. International Journal of Molecular Sciences, 24(5), 4788–4805. https://doi.org/10.3390/ijms24054788

Derbak, H., Moussaoui, M., Benberkane, A., & Ayad, A. (2021). In-vitro effect of Peganum harmala total alkaloids on spermatozoa quality and oxidative stress of epididymal ram semen. Asian Pacific Journal of Reproduction, 10(5), 232–238. https://doi.org/10.4103/2305-0500.326721

Dhurhania, C. E., & Novianto, A. (2019). Uji Kandungan Fenolik Total dan Pengaruhnya terhadap Aktivitas Antioksidan dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens). Jurnal Farması Dan Ilmu Kefarmasıan Indonesıa, 5(2), 62. https://doi.org/10.20473/jfiki.v5i22018.62-68

Furi, M., Al Basit, N., Ikhtiarudin, I., & Utami, R. (2020). Penentuan Total Fenolik, Flavonoid dan Uji Aktivitas Antioksidan Ekstrak dan Fraksi Daun Kedabu (Sonneratia ovata Backer). Jurnal Farmasi Indonesia, 12(1), 48-59

Gholami-Ahangaran, M., Karimi-Dehkordi, M., Akbari Javar, A., Haj Salehi, M., & Ostadpoor, M. (2021). A systematic review on the effect of Ginger (Zingiber officinale) on improvement of biological and fertility indices of sperm in laboratory animals, poultry and humans. Veterinary Medicine and Science, 7(5), 1959–1969. https://doi.org/10.1002/vms3.538

Hartanti, L., Ashari, A. M., & Warsidah, W. (2021). Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncaria gambir Roxb. Berkala Sainstek, 9(3), 131. https://doi.org/10.19184/bst.v9i3.27179

Hasan, H., Ain Thomas, N., Hiola, F., & Ibrahim, A. S. (2022). Skrining Fitokimia dan Uji Aktivitas Antioksidan Kulit Batang Matoa (Pometia pinnata) Dengan Metode 1,1-Diphenyl-2 picrylhidrazyl (DPPH). Indonesian Journal of Pharmaceutical Education, 1(3), 67–73. https://doi.org/10.37311/ijpe.v2i1.10995

Hasanah, F. (2019). Skrining Fitokimia dan Formulasi Sediaan Masker Peel-Off Ekstrak Etanol Gambir (Uncaria gambir (W. Hunter) Roxb) Secara Perkolasi. BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan), 5(2), 114–122. https://doi.org/10.31289/biolink.v5i2.1757

Hutasuhut, D. A., Aspriyanto, D., & Krishnawan Firdaus, I. W. A. (2022). Uji Fitokimia Kualitatif dan Kuantitatif Ekstrak Kulit Buah Rambai (Baccaurea Motleyana) Konsentrasi 100%. Dentin, 6(2), 97–102. https://doi.org/10.20527/dentin.v6i2.6394

Jeschke, J. K., Biagioni, C., Schierling, T., Wagner, I. V., Börgel, F., Schepmann, D., Schüring, A., Kulle, A. E., Holterhus, P. M., von Wolff, M., Wünsch, B., Nordhoff, V., Strünker, T., & Brenker, C. (2021). The Action of Reproductive Fluids and Contained Steroids, Prostaglandins, and Zn2+ on CatSper Ca2+ Channels in Human Sperm. Frontiers in Cell and Developmental Biology, 9(699554), 1–16. https://doi.org/10.3389/fcell.2021.699554

Karabulut, S., Korkmaz, O., Altun, C. E., Zergeroğlu, A. D., & Keskin, İ. (2020). Quercetin enhances human sperm motility in a dose and time dependent manner. Acta Pharmaceutica Sciencia, 58(2), 170–178. https://doi.org/10.23893/1307-2080.APS.05810

Kartika, L., Ardana, M., & Rusli, R. (2020). Aktivitas Antioksidan Tanaman Artocarpus. Proceeding of Mulawarman Pharmaceuticals Conferences, 12, 237–244. https://doi.org/10.25026/mpc.v12i1.432

Kedechi, S., Zribi, N., Louati, N., Menif, H., Sellami, A., Lassoued, S., Ben Mansour, R., Keskes, L., Rebai, T., & Chakroun, N. (2017). Antioxidant effect of hydroxytyrosol on human sperm quality during in vitro incubation. Andrologia, 49(1), e12595. https://doi.org/10.1111/and.12595

Minhas, S., Bettocchi, C., Boeri, L., Capogrosso, P., Carvalho, J., Cilesiz, N. C., Cocci, A., Corona, G., Dimitropoulos, K., Gül, M., Hatzichristodoulou, G., Jones, T. H., Kadioglu, A., Martínez Salamanca, J. I., Milenkovic, U., Modgil, V., Russo, G. I., Serefoglu, E. C., Tharakan, T., … Salonia, A. (2021). European Association of Urology Guidelines on Male Sexual and Reproductive Health: 2021 Update on Male Infertility. European Urology, 80(5), 603–620. https://doi.org/10.1016/j.eururo.2021.08.014

Permatasari, S., Bahnar, S., & Pujianto, D. A. (2023). Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility. Jurnal Kedokteran Dan Kesehatan Indonesia, 14(2), 128–134. https://doi.org/10.20885/JKKI.Vol14.Iss2.art3

Permatasari, S., Frethernety, A., & Shinta, H. E. (2023). The transfluthrin effect on mosquito repellent to sperm quality and testicular histology of rats. The 8th Internatıonal Conference and Workshop on Basıc and Applıed Scıence (ICOWOBAS) 2021, 2554, 1–2. https://doi.org/10.1063/5.0103917

Piomboni, P., Focarelli, R., Stendardi, A., Ferramosca, A., & Zara, V. (2012). The role of mitochondria in energy production for human sperm motility. International Journal of Andrology, 35(2), 109–124. https://doi.org/10.1111/j.1365-2605.2011.01218.x

Proklamasiningsih, E., Budisantoso, I., & Maula, I. (2019). Pertumbuhan Dan Kandungan Polifenol Tanaman Katuk (Sauropus androgynos (L.) Merr) Pada Media Tanam Dengan Pemberian Asam Humat. Al-Kauniyah: Jurnal Biologi, 12(1), 96–102. https://doi.org/10.15408/kauniyah.v12i1.8972

Pujianto, D., Sisca, & Yunaini, L. (2019). Progesterone increases capacitation and exerts a prosurvival effect in sperm via Akt activation. Journal of Natural Science, Biology and Medicine, 10(3), S24–S28.

Puspitaningrum, D., & Nugraheni, S. (2022). Determinants of Male and Female Infertility: A Systematic Review. Jurnal Kebidanan, 11(2), 103. https://doi.org/10.26714/jk.11.2.2022.103-120

Quyen, L. T., Thu, N. T. M., Phuong, L. T. M., & Trang, N. T. (2020). Comparison of the Sssperm Testing Kit with the Halosperm Testing Kit in the Analyzing Sperm DNA Fragmentation. London Journal of Medical and Health Research, 20(2), 17–25.

Rollando, R., Ardanareswari, A., Susanto, F. H., & Monica, E. (2022). Efek Afrodisiaka dari Ekstrak Batang Bajakah Kalalawit (Uncaria gambir Roxb) terhadap Tikus Jantan Galur Wistar (Rattus novergicus). Jurnal Pharmascience, 9(2), 213. https://doi.org/10.20527/jps.v9i2.13289

Sari, R. M., Rita, R. S., & Anas, E. (2018). Pengaruh Pemberian Isolat Katekin Gambir (Uncaria gambir Roxb) Terhadap Kadar Hormon Testosteron Dan Jumlah Spermatozoa Tikus Rattus Norvegicus Jantan Hiperglikemia. Jurnal Kesehatan Andalas, 7(3), 6–9. http://jurnal.fk.unand.ac.id

Silva, E. C. B., Arruda, L. C. P., Vieira, J. I. T., Soares, P. C., & Guerra, M. M. P. (2019). (+)-Catechin and (-)-epigallocatechin gallate: Are these promising antioxidant therapies for frozen goat semen? Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 71(2), 521–528. https://doi.org/10.1590/1678-4162-10539

Skibińska, I., Andrusiewicz, M., Jendraszak, M., Żbikowska, A., Jędrzejczak, P., & Kotwicka, M. (2022). Expression of estrogen receptors, PELP1, and SRC in human spermatozoa and their associations with semen quality. Human Cell, 36(2), 554–567. https://doi.org/10.1007/s13577-022-00847-6

Wagner, H., Cheng, J. W., & Ko, E. Y. (2018). Role of reactive oxygen species in male infertility: An updated review of literature. Arab Journal of Urology, 16(1), 35–43. https://doi.org/10.1016/j.aju.2017.11.001

Wehrli, L., Galdadas, I., Voirol, L., Smieško, M., Cambet, Y., Jaquet, V., Guerrier, S., Gervasio, F. L., Nef, S., & Rahban, R. (2023). The action of physiological and synthetic steroids on the calcium channel CatSper in human sperm. Frontiers in Cell and Developmental Biology, 11, 1221578–1221593. https://doi.org/10.3389/fcell.2023.1221578

Wijayanti, R., Wahyuono, S., Sari, I. P., & Rizal, D. M. (2020). Stimulatory effect of methanolic extract and N-hexane insoluble and soluble fraction of parijoto fruit (Medinilla speciosa Blume) on the spermatozoa quantity of male Sprague Dawley rats. In Medical Technology and Environmental Health (pp. 9–13). https://doi.org/10.1201/9781003016700-2

World Health Organization. (2021). WHO laboratory manual for the examination and processing of human semen (Sixth Edition). World Health Organization. Accessed on 11 March 2023, from https://iris. who.int/bitstream/handle/10665/343208/9789240030787-eng.pdf?sequence=1

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Published

2023-12-23

How to Cite

Permatasari, S., Halisa, N., & Frethernety, A. (2023). Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility. Medical Laboratory Technology Journal, 9(2). https://doi.org/10.31964/mltj.v9i2.563

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