The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty

Authors

  • Silvani Permatasari Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia
  • Alya Firyal Nuha Bachelor degree of Medical, Faculty of Medicine, Universitas Palangka Raya, Indonesia
  • Arini Ratnasari Department of Parasitology, Faculty of Medicine, Universitas Palangka Raya, Indonesia
  • Ratna Widayati Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia
  • Rian Ka Praja Department of Microbiology, Faculty of Medicine, Universitas Palangka Raya, Indonesia

DOI:

https://doi.org/10.31964/mltj.v10i1.571

Keywords:

Luvunga sarmentosa, methanol fractionation, sperm membrane integrity

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.

References

Adami, L. N. G., Maciel Junior, V. L., & Losano, J. D. (2022). Review on the role of antioxidant supplementation against oxidative stress: a human and animal approach to male fertility. Research, Society and Development, 11(1), e43211125191. https://doi.org/10.33448/rsd-v11i1.25191

Alahmar, A. (2019). Role of oxidative stress in male infertility: An updated review. Journal of Human Reproductive Sciences, 12(1), 4. https://doi.org/10.4103/jhrs.JHRS_150_18

Amelia, L., & Rahmanisa, S. (2020). Evaluasi dan Manajemen Infertilitas Pria. JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia, 7(2), 105–114. https://doi.org/10.53366/jimki.v7i2.84

Assidi, M. (2022). Infertility in Men: Advances towards a comprehensive and integrative strategy for precision theranostics. Cells, 11(10), 1–29. https://doi.org/10.3390/cells11101711

Brenda C., J. H. A. (2022). Peranan Suplemen dalam Tata Laksana Infertilitas. Cermin Dunia Kedokteran, 49(4), 193–197. https://doi.org/10.55175/cdk.v49i4.217

Check, J. H., Check, D. L., & Bollendorf, A. (2023). Hypo-Osmotic Swelling Test and Male Factor. Reproductive Medicine, 4(2), 118–132. https://doi.org/10.3390/reprodmed4020013

Derbak, H., Moussaoui, M., Benberkane, A., & Ayad, A. (2021). In-vitro effect of Peganum harmala total alkaloids on spermatozoa quality and oxidative stress of epididymal ram semen. Asian Pacific Journal of Reproduction, 10(5), 232–238. https://doi.org/10.4103/2305-0500.326721

Feng, C., Zhu, Z., Bai, W., Li, R., Zheng, Y., Tian, X., Wu, D., Lu, H., Wang, Y., & Zeng, W. (2020). Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine. Animals, 10(9), 1549. https://doi.org/10.3390/ani10091549

Grogan, S., & Preuss, C. V. (2023). Pharmacokinetics. In StatPearls. In : StatPearls. Treasure Island (FL) : StatPearls Publishing. Retrived December 7, 2023. http://www.ncbi.nlm.nih.gov/pubmed/15815736

Li, J., Wu, Y., Dong, S., Yu, Y., Wu, Y., Xiang, B., & Li, Q. (2023). Research Progress on Neuroprotective Effects of Isoquinoline Alkaloids. Molecules, 28(12), 4797. https://doi.org/10.3390/molecules28124797

Moretti, E., Signorini, C., Corsaro, R., Giamalidi, M., & Collodel, G. (2023). Human Sperm as an In Vitro Model to Assess the Efficacy of Antioxidant Supplements during Sperm Handling: A Narrative Review. Antioxidants, 12(5), 1098. https://doi.org/10.3390/antiox12051098

Neelam, Khatkar, A., & Sharma, K. K. (2020). Phenylpropanoids and its derivatives: biological activities and its role in food, pharmaceutical and cosmetic industries. Critical Reviews in Food Science and Nutrition, 60(16), 2655–2675. https://doi.org/10.1080/10408398.2019.1653822

Permatasari, S., Bahnar, S., & Pujianto, D. A. (2023). Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility. Jurnal Kedokteran Dan Kesehatan Indonesia, 14(2), 128–134. https://doi.org/https://doi.org/10.20885/JKKI.Vol14.Iss2.art3

Puspitaningrum, D., Rahfiludin, M. Z., Shaluhiyah, Z., & Winarni, S. (2022). The role of health workers and support system counselors for women with primary infertility. Jurnal Administrasi Kesehatan Indonesia, 10(2), 241–250. https://doi.org/10.20473/jaki.v10i2.2022.241-250

Syarpin, Permatasari, S., & Pujianto, D. A. (2023). Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS. Biodiversitas Journal of Biological Diversity, 24(2), 733–740. https://doi.org/10.13057/biodiv/d240208

Vardiyan, R., Ezati, D., Anvari, M., Ghasemi, N., & Talebi, A. (2020). Effect of L-carnitine on the expression of the apoptotic genes Bcl-2 and Bax. Clinical and Experimental Reproductive Medicine, 47(3), 155–160. https://doi.org/10.5653/cerm.2019.03440

Wijayanti, R., Wahyuono, S., Puspitasari, I., & Rizal, D. M. (2021). Effect of methanol extract, methanol fraction, and n-hexane fraction of parijoto fruit (medinilla speciosa) on seminal parameters and testicular weight of male sprague dawley rats. Research Journal of Pharmacy and Technology, 14(11), 5926–5930. https://doi.org/10.52711/0974-360X.2021.01030

Yendraliza, Y., Yuliana, E., Rodiallah, M., & Zumarni, Z. (2019). Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST. Jurnal Agripet, 19(1), 22–30. https://doi.org/10.17969/agripet.v19i1.13191

Yusrina, A., Solihati, N., & Hilmia, N. (2018). Pengaruh Waktu Inkubasi Pada Proses Sexing Sperma Berbasis dan Membran Plasma Utuh Chilled Semen Domba Lokal. Jurnal Ilmu Ternak Universitas Padjadjaran, 18(1), 45. https://doi.org/10.24198/jit.v18i1.17307

Zhang, W., Min, L., Li, Y., Lang, Y., Hoque, S. A. M., Adetunji, A. O., & Zhu, Z. (2022). Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation. Animals, 12(19), 2626. https://doi.org/10.3390/ani12192626

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Published

2024-06-30

How to Cite

Permatasari, S. ., Nuha, A. F., Ratnasari, A., Widayati, R., & Ka Praja, R. (2024). The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty. Medical Laboratory Technology Journal, 10(1), 11–17. https://doi.org/10.31964/mltj.v10i1.571

Issue

Vol. 10 No. 1 (2024): June

Section

Articles

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Copyright (c) 2024 Alya Firyal Nuha, Silvani Permatasari, Arini Ratnasari, Ratna Widayati, Rian Ka Praja

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