The Analgesic Effect and Toxicity of Red and White Ginger on Mus Musculus with the Acetate Writhing Test
DOI:
https://doi.org/10.31964/mltj.v10i2.611Keywords:
Analgesic, red ginger, white ginger, writhing testAbstract
Based on data from the World Health Organization in 2020, the incidence of dysmenorrhea was 1,769,425 (90%) women who suffered from dysmenorrhea, with 10-16% suffering from severe dysmenorrhea. The problem of pain is very disruptive to activities, so prevention and treatment of pain must be a concern. Ginger is a natural ingredient that grows in South Kalimantan and is empirically used to treat pain. The study aimed to determine the analgesic effects and toxicity of red and white ginger from Banjarmasin and Banjar Regency. The nutrient content profile of the soil where the ginger grew was analyzed, and it was determined by nitrogen content, cation exchange capacity, and soil pH. The content of active compound groups was analyzed using specific reagents to determine the presence of active compounds. Determination of total phenolic levels using a colorimetric method using a UV-Vis spectrophotometer. The instant ginger formula was optimized with organoleptic test result parameters. Acute toxicity tests using male Wistar rats with observations for 14 days. Analgesic tests were conducted on Mus musculus using the acetic acid writhing test. The results showed that the highest nitrogen (1,34%), cation exchange capacity (29.22 me/100 g), and soil pH levels (6,89) were in the soil where the ginger samples from Banjar Regency grew. The chemical compound content in all gingers contained the same phenolics, flavonoids, tannins, saponins, and terpenoids. The levels of marker compounds in succession from Banjarmasin red ginger, Banjarmasin white ginger, Banjar Regency red ginger, and Banjar Regency white ginger were 7.43%, 6.26%, 8.52%, and 7.12%, respectively. The results of formula optimization showed that all gingers had a sweet and spicy taste, a distinctive odor, powder form, and a reddish-brown color in red ginger and pale yellow in white ginger. The toxicity test results showed that at doses of 300 mg/Kg BW and 2000 mg/Kg BW, there was no toxic effect on the administration of ginger extract. Analgesic activity showed that all gingers had an analgesic effect at a dose of 200 mg/Kg BW. The analgesic effects from the strongest to the lowest were red ginger from Banjar Regency, red ginger from Banjarmasin, white ginger from Banjar Regency, and white ginger from Banjarmasin. This study concludes that red ginger from Banjar Regency has the most potent analgesic activity and no toxicity for all ginger.References
Adawiyah, R., & Rizki, M. I. (2018). Aktivitas Antioksidan Ekstrak Etanol Akar Kalakai (Stenochlaena palustris Bedd) Asal Kalimantan Tengah. Jurnal Pharmascience, 5(1), 71–77. https://doi.org/10.20527/jps.v5i1.5788
Akbar, N., & Rizki, M. I. (2015). Potential Analgesic Agents and Action Mechanism of Phytochemicals from Indonesia Natural Products – A Review. Proceedings of International Conference on Names, 117–123.
Akhmadi, F. (2024). Analisis Dampak Hilirisasi Terhadap Pertumbuhan Ekonomi Di Indonesia. Hatta: Jurnal Pendidikan Ekonomi dan Ilmu Ekonomi, 2(1), 25–31. https://doi.org/10.62387/hatta.v2i1.18
Bahrudin, M. (2018). Patofisiologi Nyeri (Pain). Saintika Medika, 13(1), 7. https://doi.org/10.22219/sm.v13i1.5449
BPOM RI. (2022). Peraturan Badan Pengawas Obat dan Makanan Nomor 10 Tahun 2022 Tentang Pedoman Uji Toksisitas Praklinik Secara In Vivo. BPOM RI.
Dewi, S. R., & Salim, H. (2018). Uji Efek Analgetik Infusa Jahe (Zingiber officianale Roscoe) Terhadap Hewan Uji Mencit Jantan (Mus musculus). Media Farmasi, 14(2), 15. https://doi.org/10.32382/mf.v14i2.585
Effendi, D. S. (2000). Identifikasilahan Bagi Pengembangan Tanaman Jahe (Zingiber offlcinale Rose.) dan Melinjo (Gnetum gnemon L.). Berita Biologi, 5(2), 231–237.
Farisi, S. A., Munawir, A., & Febianti, Z. (2015). Uji Toksisitas Akut Ekstrak Buah Bruguiera gymnorrhiza pada Tikus (Rattus norvegicus). E-Jurnal Pustaka Kesehatan, 3(2), 230–234.
Goenarwo, E., Chodidjah, C., & Susanto, H. (2011). The Trial on the Effectiveness of Honey on White Rats Using Geliate Acetate Method An Experimental Study in Male Wistar Rat. Sains Medika : Jurnal Kedokteran dan Kesehatan, 3(1), 48. https://doi.org/10.30659/sainsmed.v3i1.408
Kaunang, C. E., Bodhi, W., & Edi, H. J. (2020). Uji Efek Analgetik Nanopartikel Ekstrak Rimpang Jahe Merah (Zingiber officinale Var Rubrum) Pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus). Pharmacon, 9(2), 184–193. https://doi.org/10.35799/pha.9.2020.29269
Kuna, L., Jakab, J., Smolic, R., Raguz-Lucic, N., Vcev, A., & Smolic, M. (2019). Peptic Ulcer Disease: A Brief Review of Conventional Therapy and Herbal Treatment Options. Journal of Clinical Medicine, 8(2), 179. https://doi.org/10.3390/jcm8020179
Lestari, I., Hakiki, N., Nurjanah, S., Jamil, T. K., & Sativa, N. (2024). Karakter Morfologi dan Hubungan Kekerabatan pada Tanaman Jahe (Zingiber officinale) di Kabupaten Garut. Jurnal Sumberdaya Hayatı, 10(3), 150–156.
Mao, Q.-Q., Xu, X.-Y., Cao, S.-Y., Gan, R.-Y., Corke, H., Beta, T., & Li, H.-B. (2019). Bioactive Compounds and Bioactivities of Ginger (Zingiber officinale Roscoe). Foods, 8(6), 185. https://doi.org/10.3390/foods8060185
Nopsagiarti, T., Okalia, D., & Marlina, G. (2020). Analisis C-Organik, Nitrogen dan C/N Tanah Pada Lahan Agrowisata Beken Jaya. Jurnal Agrosains dan Teknologi, 5(1), 11–18.
Pattiiha, N., Novelia, S., & Suciawati, A. (2021). Pengaruh Air Kelapa Muda Hijau Terhadap Nyeri Dismenore Pada Remaja. Indonesian Jurnal of Health Development, 3(1), 231–238.
Rizki, M. I., Nurlely, N., Fadlilaturrahmah, F., & Ma’shumah, M. (2021a). Antioxidant activities of ethanol extract leaves of nangka (Artocarpus heterophyllus), cempedak (Artocarpus integer), and tarap (Artocarpus odoratissimus) from south kalimantan. Journal of Current Pharmaceutical Sciences, 4(2), 367–372.
Rizki, M. I., Nurlely, N., Fadlilaturrahmah, F., & Ma’shumah, M. (2021b). Skrining Fitokimia dan Penetapan Kadar Fenol Total Pada Ekstrak Daun Nangka (Artocarpus heterophyllus), Cempedak (Artocarpus integer), dan Tarap (Artocarpus odoratissimus) Asal Desa Pengaron Kabupaten Banjar. Jurnal Insan Farmasi Indonesia, 4(1), 95–102. https://doi.org/10.36387/jifi.v4i1.667
Rizki, M. I., Sari, A. K., Kartika, D., Khairunnisa, A., & Normaidah. (2022). Penetapan kadar fenolik total dan uji aktivitas antioksidan fraksi dari ekstrak etanol daun cempedak (Artocarpus integer) dengan metode DPPH. MPI (Media Pharmaceutica Indonesiana), 4(2), 168–178.
Sa’diah, S., Anwar, E., Jufri, M., & Cahyaningsih, U. (2019). Perbandingan Ekstrak Jahe Merah (Zingiber Officinale Roscoe. Var. Rubrum), Gingerol dan Shogaol sebagai Anti-Toksoplasma terhadap Parasit Toxoplasma Gondii Secara In-Vitro. Jurnal Jamu Indonesia, 4(3), 93–102. https://doi.org/10.29244/jji.v4i3.160
Sandy, P. M., & Susilawati, Y. (2021). Review Artikel: Manfaat Empiris dan Aktivitas Farmakologi Jahe Merah (Zingiber officinale Roscoe), Kunyit (Curcuma domestica Val.) dan Kencur (Kaempferia galanga L.). Farmaka, 19(2), 36–47.
Sari, A. K., Aisyah, N., & Prihandiwati, E. (2020). Penentuan kadar fenolik total ekstrak etanol 96% daun terap (Artocarpus odoratissimus Blanco) dengan metode spektrofotometri visibel. Jurnal Ilmiah Ibnu Sina, 5(1), 171–179.
Sari, D., & Nasuha, A. (2021). Kandungan Zat Gizi, Fitokimia, dan Aktivitas Farmakologis pada Jahe (Zingiber officinale Rosc.): Review. Tropical Bioscience: Journal of Biological Science, 1(2), 11–18. https://doi.org/10.32678/ tropicalbiosci.v1i2.5246
Sartika, L., Desnita, R., & Isnindar. (2022). Potensi Kombinasi Jahe (Zingiber officinale Rosc). Dan Temulawak (Curcuma xanthorrhiza Roxb.) Sebagai Minuman Kesehatan. Jurnal Mahasiswa Farmasi Fakultas Kedokteran Untan, 6(1), 1–10.
Shawahna, R., & Taha, A. (2017). Which Potential Harms and Benefits of Using Ginger in the Management of Nausea And Vomiting of Pregnancy Should Be Addressed? A Consensual Study Among Pregnant Women and Gynecologists. BMC Complementary and Alternative Medicine, 17(1), 204. https://doi.org/10.1186/s12906-017-1717-0
Srikandi, S., Humaeroh, M., & Sutamihardja, R. (2020). Kandungan Gingerol Dan Shogaol Dari Ekstrak Jahe Merah (Zingiber Officinale Roscoe) Dengan Metode Maserasi Bertingkat. al-Kimiya, 7(2), 75–81. https://doi.org/10.15575/ ak.v7i2.6545
Sukweenadhi, J., Damitasari, P. D., Kartini, K., Christanti, P., & Putri, E. N. (2023). Gingerol and Shogaol on Red Ginger Rhizome (Zingiber officinale Var. Rubrum) using High-Performance Liquid Chromatography. Pharmaciana, 13(2), 166. https://doi.org/10.12928/pharmaciana.v13i2.25246
Suryani, I. (2014). Kapasitas Tukar Kation (KTK) Berbagai Kedalaman Tanah Pada Areal Konversi Lahan Hutan. Jurnal Agrisistem, 10(2), 99–106.
Syaputri, E., Selaras, G., & Farma, S. (2021). Manfaat Tanaman Jahe (Zingiber officinale) Sebagai Obat obatan Tradisional (Traditional Medicine). Prosiding Semnas Bıo, 1, 579–586.
Tsamara, G., Raharjo, W., & Putri, E. A. (2020). Hubungan Gaya Hidup dengan Kejadian Dismenore Primer pada Mahasiswi Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Tanjungpura. Jurnal Nasional Ilmu Kesehatan, 2(3), 130–140.
Wibawa, I. P. A. H., & Tirta, I. G. (2021). Aktivitas Antioksidan Ekstrak Methanol Jahe Merah (Zingiber officinale var. Rubrum Theilade), Bayam Hias Merah (Iresine herbstii Hook.) dan Azolla Merah (Azolla pinnata R. Br.). Jurnal Wıdya Bıologı, 12(02), 77–80. https://doi.org/10.32795/widyabiologi.v12i02.2140
Yuliani, S. S., Useng, D., & Achmad, M. (2017). Analisis Kandungan Nitrogen Tanah Sawah Menggunakan Spektrometer. Jurnal Agritechno, 10(2), 188–202. https://doi.org/10.20956/at.v10i2.71
Yulianti, F., & Susilowati, T. (2022). Gambaran Dismenorea Saat Aktivitas Belajar Diruang Kelas Pada Siswi Sma Muhammadiyah 1 Sragen. Journal Locus Penelitian dan Pengabdian, 1(6), 459–465. https://doi.org/10.36418/locus.v1i6. 143
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