AKURASI HITUNG JUMLAH ERITROSIT METODE MANUAL DAN METODE OTOMATIS

Neni Oktiyani, fahriyan fahriyan, Akhmad Muhlisin

Abstract

In a laboratory, an erythrocyte is done by using hemocytometer and microscope. The task is to measure and assess the size and shape of the erythrocyte. But this procedure is time consuming, complex and tedious. As a solution to this problem, to provide an automated, cost-effective and efficient alternative to detection and counting of erythrocyte, hematology analyzers are used. However, false results related either to erythrocyte or other parameters from complete blood count may be observed in several instances. The objective study was to compare the accuracy of erythrocyte count results of automatic hematological analysis by the manual method. The study is an analytic survey with a cross-sectional design. Erythrocyte counting is done by using three types of control blood, namely high, normal and low, with 9 repetitions. In high control blood, the mean erythrocytes count by the manual method was 7.08 million/μl with a bias value of 1.4%, while that by the automated method was 7.03 million/μl with a bias value of 0.7%. The mean erythrocyte count in normal control blood by the manual method was 4.50 million/μl with a bias value of 0.9%, while that by the automated method was 4.4 million/μl with a bias value of 2.4%. And in low control blood, the mean erythrocyte count by the manual method was 1.72 million/μl with 4.4% bias value, while that the automated method was 1.67 million/μl with 1.2% bias value. Statistical analysis showed no significant difference in accuracy between the erythrocyte count of manual methods with automated methods.

Hitung jumlah eritrosit dilakukan dengan menggunakan metode manual dan metode otomatis. Metode manual menggunakan alat Hemositometer dapat memberikan hasil yang dipercaya dan akurat tergantung keahlian dari teknisi laboratorium. Metode otomatis memberikan hasil yang lebih mudah, cepat dan teliti dibandingkan dengan cara manual. Tujuan penelitian untuk membandingkan akurasi hitung jumlah eritrosit metode manual dan metode otomatis. Jenis penelitian yang digunakan adalah survey analitik dengan rancangan cross sectional. Penelitian ini membandingkan data hasil hitung jumlah eritrosit antara metode manual dengan metode otomatis dengan menggunakan 3 jenis darah kontrol hitung jumlah eritrosit high, normal, dan low dengan 9 kali pengulangan. Hasil penelitian menunjukkan nilai rerata hitung jumlah eritrosit darah kontrol high metode manual sebesar 7,08 juta/µl dengan nilai bias 1,4% dan metode otomatis sebesar 7,03 juta/µl dengan nilai bias 0,7%. Rerata hitung jumlah eritrosit darah kontrol normal metode manual sebesar 4,50 juta/µl dengan nilai bias 0,9% dan metode otomatis sebesar 4,43 juta/µl dengan nilai bias 2,4%. Rerata hitung jumlah eritrosit darah kontrol low metode manual sebesar 1,72 juta/µl dengan nilai bias 4,4% dan metode otomatis sebesar 1,67 juta/µl dengan nilai bias 1,2%. Uji statistik menunjukkan tidak ada perbedaan akurasi yang bermakna antara hitung jumlah eritrosit metode manual dengan metode otomatis.

Keywords

hitung jumlah eritrosit; manual; otomatis

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