Effect of Vitamin C Exposure to Fibroblast Cells on Woman Uterosacral Ligament Culture
Keywords:Fibroblast, pelvic organ prolapse, uterosacral ligament culture, vitamin C
AbstractPelvic Organ Prolapse (POP) occurs when the uterosacral ligaments weaken. Vitamin C plays a major role in forming the extracellular matrix (collagen) and stimulates fibroblast cell proliferation. This study aimed to examine how a woman's uterosacral ligament culture was affected by vitamin C exposure to fibroblast cells. A straightforward experimental design investigation that is non-blind and includes analysis after exposure (Post-test control group design). This study's sample used uterosacral ligament from a woman who underwent at least two vaginal deliveries through a hysterectomy procedure. Several doses of vitamin C were given and repeated for 72 hours, followed by examining the number of fibroblast cells; then, the data was analyzed starting with the parametric prerequisite test and the one-way ANOVA test. There was a significant effect of vitamin C exposure on fibroblast cells of the uterosacral ligament at a dose of 50 μg/ml and 100 μg/ml. In contrast, at doses of 150 μg/ml and 200 μg/ml, there was no significant effect. From this study, it is hoped that the administration of vitamin C according to the optimal dose found in the study (significant impact at doses of 50 μg/ml and 100 μg/ml, can increase the proliferation of fibroblast matrix supporting the sacrouterine ligament in women, to prevent prolapse of the pelvic organs in women, and can maintain the quality of life of women.
Adnyana, I. G. H. E., Suwiyoga, K., & Megadhana, W. (2018). Ekspresi kolagen I, elastin, dan ketebalan epitel dinding vagina paskamenopause lebih rendah dibandingkan premenopause. Medicina, 49(2) 31-35.
Carr, A. C., & Maggini, S. (2017). Vitamin C and immune function. Nutrients, 9(11), 1211.
Connell, K. A., Guess, M. K., Chen, H. W., Lynch, T., Bercik, R., & Taylor, H. S. (2009). HOXA11 promotes fibroblast proliferation and regulates p53 in uterosacral ligaments. Reproductive Sciences, 16, 694–700.
De la Fuente, M., Sánchez, C., Vallejo, C., Díaz-Del Cerro, E., Arnalich, F., & Hernanz, Á. (2020). Vitamin C and vitamin C plus E improve the immune function in the elderly. Experimental Gerontology, 142, 111118.
Di Tano, M., Raucci, F., Vernieri, C., Caffa, I., Buono, R., Fanti, M., Brandhorst, S., Curigliano, G., Nencioni, A., & de Braud, F. (2020). Synergistic effect of fasting-mimicking diet and vitamin C against KRAS mutated cancers. Nature Communications, 11(1), 2332.
Dixon, M. W. (2007). Myofascial Massage. Lippincott Williams & Wilkins: Baltimore
Freshney, R. I. (2010). Animal cell culture: a practical approach . Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. John Wiley & Sons, Inc: Hoboken, New Jersey
Hutabarat, Y., Megadhana, I. W., Suwiyoga, K., Astawa, N. M., & Arthawan, A. (2020). Ekspresi sel fibroblas yang rendah pada ligamentum sakrouterina sebagai faktor risiko terjadinya prolaps uterus derajat III-IV di RSUP Sanglah Denpasar, Bali-Indonesia. Intisari Sains Medis, 11(2), 830–834.
Jin, J., Jia, Z.-H., Luo, X.-H., & Zhai, H.-F. (2020). Long non-coding RNA HOXA11-AS accelerates the progression of keloid formation via miR-124-3p/TGFβR1 axis. Cell Cycle, 19(2), 218–232.
Junqueira, L. C. U., & Carneiro, J. (2005). Basic histology: text & atlas (Vol. 3). McGraw-Hill New York.
Malole, M. B. (1990). Kultur sel dan jaringan hewan. Pusat Antar Bogor: Universitas. Institut Pertanian Bogor.
Mohammed, B. M., Fisher, B. J., Kraskauskas, D., Ward, S., Wayne, J. S., Brophy, D. F., Fowler III, A. A., Yager, D. R., & Natarajan, R. (2016). Vitamin C promotes wound healing through novel pleiotropic mechanisms. International Wound Journal, 13(4), 572–584.
Mubarak, N. M., Anwar, M., Debnath, S., & Sudin, I. (2023). Basic Human Biology in Terms of Anatomy and Histology. In Fundamentals of Biomaterials: A Supplementary Textbook (pp. 115–121). Springer.
Pack, E., Stewart, J., Rhoads, M., Knight, J., Clark, S., Schmale III, D. G., & De Vita, R. (2020). Effects of short-term moderate ZEN consumption on uterosacral ligament elasticity in pubertal gilts. Research in Veterinary Science, 133, 202–209.
Ronchetti, I. P., Quaglino Jr, D., & Bergamini, G. (1996). Ascorbic acid and connective tissue. Subcellular Biochemistry: Ascorbic Acid: Biochemistry and Biomedical Cell Biology, 25(13), 249–264.
Su, X., Ma, Y., Wang, Q., & Gao, Y. (2021). LncRNA HOXA11-AS aggravates keloid progression by the regulation of HOXA11-AS-miR-205-5p-FOXM1 pathway. Journal of Surgical Research, 259, 284–295.
Sugiritama, I. W. (2023). Efektivitas Fitoestrogen dalam Mengurangi Gejala Atrofi Vagina Pada Wanita Menopause. Media Sains Indonesia,7(9), 22-26
Uehara, H., Itoigawa, Y., Morikawa, D., Koga, A., Tsurukami, H., Maruyama, Y., & Ishijima, M. (2023). The Effect of Vitamin C and N-Acetylcysteine on Tendon-to-Bone Healing in a Rodent Model of Rotator Cuff Repair. The American Journal of Sports Medicine, (1-3), 209-21703635465231160772.
Vasanth, S., Dubey, A., GS, R., Lewis, S. A., Ghate, V. M., El-Zahaby, S. A., & Hebbar, S. (2020). Development and investigation of vitamin C-enriched adapalene-loaded transfersome gel: a collegial approach for the treatment of acne vulgaris. AAPS PharmSciTech, 21, 1–17.
Vishniakova, K. S., Kireev, I. I., & Dunina-Barkovskaya, A. Y. (2011). Effects of cell culture density on phagocytosis parameters in IC-21 macrophages. Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 5, 355–363.
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Copyright (c) 2023 Rahajeng Rahajeng, Tatit Nurseta, Edy Mustofa, Yahya Irwanto, Aditiya Fendi Uji Pamungkas, Dhian Eka Putri Harnandari, Putu Arik Herliawati
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