Effect of Papaya Leaf Extract Gel (Carica papaya) on Incision Wound Healing in Rattus norvegicus
Keywords:Incision wounds, papaya leaf extract gel, wound healing
AbstractCarica papaya is one kind of herbal plant that can help the wound healing process. Scientifically, the leaves of papaya are proven to have anti-inflammatory effects. Flavonoid, alkaloid, tannin, and saponin compounds in papaya leaves have antimicrobial and antioxidant properties to accelerate the re-epithelization and cell migration needed for wound healing. This research aims to determine the effect of papaya leaf extract gel (Carica papaya) on the healing of incision wounds in Rattus norvegicus based on changes in wound morphology (signs of redness, edema, pus, granulation, moisture, and wound length). This research used female Rattus norvegicus aged 12-16 weeks weighing 180-200 grams, divided into five groups (NaCl 0,9%, povidone-iodine, papaya leaf extract gel with concentrations of 10%, 20%, and 30%). Incision wounds on the back of the rat with a length of 2 cm and depth of 1 cm. The normality test used the Shapiro-Wilk test, and the homogeneity test used the Levene test. The research data were not normally distributed nor homogeneous, so they were analyzed using the Kruskal Wallis and Mann-Whitney tests. The results showed that the extract of papaya leaves with concentrations of 10%, 20%, and 30% had significant differences in positive and negative control groups in the wound healing process with the differences in redness (p=0.038), granulation tissue (p=0.039), moistures (0,48), and wound length (p=0,049). It can be concluded that papaya leaf extract gel (Carica papaya) is effective in accelerating the healing process of incision wounds in Rattus norwegicus at concentrations of 10%, 20%, and 30%, so for further research, it is hoped that a histopathological examination can be carried out so that changes that occur in collagen, neutrophile cells, monocytes, and lymphocytes are seen.
Dewi, A. U., & Wıcaksono, I. A. (2020). Tanaman Herbal yang Memiliki Aktivitas Penyembuhan Luka. Farmaka, 18(2), 191-207.
Femilian, A., Agustina, D., & Subagyo, G. (2019). The Effect of Papaya Leaf Extract (Carica Papaya L) on Healing Process of Buccal Traumatic Ulcer in Wistar Rats. Majalah Kedokteran Gigi Indonesia, 5(1), 15-22.
Kaur, M., Talniya, N. C., Sahrawat, S., Kumar, A., & Stashenko, E. E. (2019). Ethnomedicinal Uses, Phytochemistry and Pharmacology of Carica Papaya Plant: A Compendious Review. Mini-Reviews in Organic Chemistry, 16(5), 463-480.
Khor, B. K., Chear, N. J. Y., Azizi, J., & Khaw, K. Y. (2021). Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica Papaya Leaf Extracts A Comparison of Supercritical Fluid and Conventional Extraction Methods. Molecules, 26(5), 1489.
Kumar, S., Kumar, A., Kumar, R., Kumar, V., Kumar, N., & Tyagi, A. (2022). Phytochemical, Pharmacognostical and Pharmacological Activities of Carica papaya. International Journal for Research in Applied Sciences and Biotechnology, 9(2), 310-315.
Nafiu, A. B., Alli-Oluwafuyi, A. M., Haleemat, A., Olalekan, I. S., & Rahman, M. T. (2019). Papaya (Carica papaya L., pawpaw). In Nonvitamin and Nonmineral Nutritional Supplements (pp. 335-359). Academic Press.
Niveditha, N. B., Sarojini, B. K., & Shrungeswara, A. H. (2020). A Study on the Synergistic Effect of Bioactive Compounds from Papaya Leaf and Collagen Isolated from Indian Mackerel on Vitro Wound Closure Capacity. Journal of Fisheries and Environment, 44(2), 24-36.
Oktaviani, D. J., Widiyastuti, S., Maharani, D. A., Amalia, A. N., Ishak, A. M., & Zuhrotun, A. (2019). Bahan Alami Penyembuh Luka. Majalah Farmasetika, 4(3), 45-56.
Santı, T. D., Sıregar, T. N., Sutrıana, A., Andını, R., & Candra, A. (2022). Phytochemical Test and Optimization of Transdermal Patches of Carica Papaya Extract: Formulation Design of Candidate Drug for Wound Healing. Biodiversitas Journal of Biological Diversity, 23(6).
Siahaan, A. V., & Chan, A. (2018). Efektivitas Sediaan Gel dari Ekstrak Etanol Daun Pegagan (Centellaasiacita L) dan Daun Pepaya (Carica papaya L. Jurnal Dunia Farmasi, 2(2), 59-69.
Sidat, P. S., Varachia, A. I., Vanshiya, S. K., Unagar, A. H., Akbari, M. G., & Dhamat, R. C. (2020). Carica papaya Leaves: One of Dynamic Plant Parts Having Multiple Therapeutic Activities. Himalayan Journal of Health Sciences, 37-48.
Sultana, A., Khan, A., Afroz, R., Yasmeen, O., Aktar, M. T., & Yusuf, M. A. (2018). Comparison of Anti-Inflammatory Effect of Ethanolic Extract of Carica Papaya Leaves and Indomethacin in Carrageenan Induced Rat Paw Edema Animal Model. Journal of Science Foundation, 16(2), 49-53.
Sumintarti, S., & Juliana, J. (2020). Effect of Cats Tail Leaves Extract (Acalypha Hispida Burm. f.) on Wound Healing (Traumatic Ulcer) of Wistar Male Rat Oral Mucosa (Rattus norvegicus). Journal of Dentomaxillofacial Science, 5(1), 56-61.
Suratmini, P. A., Wardani, I. G. A. A. K., & Suena, N. M. D. S. (2021). Potensi Tanaman Obat terhadap Epitelisasi dalam Penyembuhan Luka Bakar. Usadha: Jurnal Integrasi Obat Tradisional, 1(1).
Wadekar, A. B., Nimbalwar, M. G., Panchale, W. A., Gudalwar, B. R., Manwar, J. V., & Bakal, R. L. (2021). Morphology, Phytochemistry and Pharmacological Aspects of Carica papaya, an Review. GSC Biological and Pharmaceutical Sciences, 14(3), 234-248.
Watung, E. J., Maarisit, W., Sambou, C. N., & Kanter, J. W. (2020). Uji Efektivitas Sediaan Gel Ekstrak Batang Pepaya (Carica papaya L.) sebagai Penyembuh Luka Sayat pada Tikus Putih (Rattus novergicus). Biofarmasetikal Tropis, 3(2), 1-7.
Wijaya, D. P., Herlina, H., Fitri, N. A., Mardiyanto, M., Mustikasanti, M., & Firnando, F. (2020). Preparation, Characterization and Wound Healing Activity of Papaya Leaves Extract on Spray Gel. Majalah Obat Tradisional, 25(2), 105-109.
Winarjo, G. A., Arifin, F., & Oenarta, D. G. (2021). Comparison of the Effectiveness of Giving Binahong Leaf (Anredera cordifolia (Ten) Steenis) and Papaya Leaf (Carica papaya) on Skin Wound Healing in White Rat (Rattus novergicus). Journal of Widya Medika Junior, 3(2), 78-85.
Yadav, S., Mishra, A. P., Kumar, S., Negi, A., & Maurya, V. K. (2021). Herbal Wound Healing Agents. In Preparation of Phytopharmaceuticals for the Management of Disorders (pp. 169-184). Academic Press.
How to Cite
Publishing your paper with Medical Laboratory Technology Journal (MLTJ) means that the author or authors retain the copyright in the paper. MLTJ granted an author(s) rights to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by MLTJ in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.
MLTJ journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially. MLTJ journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:
BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.SA: If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.